Comparison of the effects of ethinyl estradiol and genistein on serum lipids and lipoproteins of hypercholestrolemic male rats

Introduction: Estrogen is one of the female sex hormones that in addition to its reproductive role, has favorable fects on the metabolism of blood lipids and lipoproteins and by this way decreases the coronary heart disease. n the other hand, phytoestrogens are phytochemical substances that have effects like estrogens. Isoflavones, hich the most important of them is genistein, are the most common form of phytoestrogens. In this research, the fects of one phytoestrogenic compounds named genistein and one estrogenic compound named ethinyl estradiol ere compaired. The purpose of this comparison was to study of replacement possibility of phytoestrogens in RT period for elimination of side effects of estrogenic compound. Methods: First the rats were fed for 2 months with high cholestrol diet (2% cholestrol, 0/5% cholic acid). fter the first blood test ensured the hypercholestrolemic condition, the rats were treated for 2 months with ethinyl stradiol and genistein besides of high cholestrol diet. After the end of the second period and serum preparation om the blood samples disignated total cholestrol, triglyceride, HDL, LDL and VDL were measured and the sults were statistically analyzed. Results: According to the obtained results of the first period of experiment, the usage of high cholestrol diet aused meaningful increase in total cholestrol and LDL as it made rats hypercholestrolemic. According to the sults of second period of experiment, the usage of ethinyl estradiol reduced the level of serum total cholestrol nd LDL of hypercholestrolemic rats, that was because of the effect of estrogen on increase of LDL catabolism. hereas, it did not produce meaningful changes in triglyceride, HDL and VDL. Usage of genistein in isolated rm had no benefical effect on blood lipids and lipoproteins, Because hypocholestrolemic property of isoflavone epends on matrix that is beside it.